Desorption of microorganisms with hydrophobized surfaces

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Mrs I.A. Bogdanova (Irkutsk State University)Mrs E.Yu. Konovalova (Irkutsk State University)


One of the most promising areas in the fight against oil pollution is the use of microorganisms. When eliminating oil pollution, an important task is the selection of surfactants that facilitate the interaction of oil-oxidizing microorganisms with oil products. Dispersion of hydrophobic substances, in particular petroleum hydrocarbons, dramatically increases their surface. In turn, this significantly increases the area of interaction of hydrophobic compounds with oxygen and oil-oxidizing microorganisms. In this work, the effect of humic substances on the aggregate state of various hydrophobic substances is studied. The bioagents were taken strains: Yarrowia lipolytica and Bacillus thuringiensis. Sodium dodecyl sulfate, melanin and humates were chosen as classic surfactants. As sorbing surfaces, slides that were pretreated with silicone or paraffin were used in the work. Experiments have shown that in the presence of sodium dodecyl sulfate, the sorption of cells and spores of microorganisms significantly decreased. With longer exposures, the concentration of cells and spores on the glasses not only did not increase, but even decreased. Moreover, the higher the content of sodium dodecyl sulfate, the weaker the cells and spores of the microorganisms were bound to the hydrophobic surface, and the rate of desorption was higher. It was shown that humates at certain concentrations, like twin, dispersed diesel fuel and fuel oil, and also reduced the amount of cell adhesion.

The work was carried out with the financial support of RFBR grant 18-48-030019 "Study of the interaction of individual strains and microbial associations possessing electrogenic activity in MFC, with pollutants of domestic wastewater and development of recommendations on intensification of their treatment". The authors are grateful to Professor B. N. Ogarkov for the kindly provided preparation of melanin, N. Prilutskaya for assistance in conducting experiments.

Affiliation of speaker Irkutsk State University
Position of speaker lecturer
Publication IOP Conference Series: Earth and Environmental Science

Primary authors

Mrs I.A. Bogdanova (Irkutsk State University) Mrs E.Yu. Konovalova (Irkutsk State University) Mr A.B. Kupchinskiy (Baikal Museum of the Irkutsk Science Center ) Ms G. O. Zhdanova (Irkutsk State University)

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